Spatial transcriptomics (ST) is a cutting-edge technology that combines microscopic imaging and sequencing techniques to capture gene expression data while preserving the spatial location of the genes within tissues. Such information provides a solid foundation for mechanistic understanding of many biological processes in both health and disease that cannot be obtained by using traditional technologies. This webinar offers a comprehensive overview of different ST methodologies coupled with a walkthrough of the ST pipeline developed by Strand. Viewers will be guided through various techniques and tools used in the workflow, providing a step-by-step overview of data acquisition, preprocessing, analysis, and interpretation. Join us as we delve into the spatial transcriptomics pipeline to unlock the potential of spatial gene expression analysis.